Prophenoloxidase Activation Pathway and its Role in Freshwater Crab, Barytelphusa Cunicularis (West-wood) Immunity
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چکیده
The prophenoloxidase activating system (proPO-AS) in fresh water crab, Barytelphusa cunicularis is localized in the hemocytes. Using anticoagulant (EDTA-Citrate buffer) it was possible to isolate intact hemocytes without causing extensive degranulation & subsequent clotting. Activation and conversion of prophenoloxidase to phenoloxidase(PO) was carried out by serine proteinase. PO was found to have molecular range between 60-70kDa. PO showed increased activity in the presence of elicitors such as β-1, 3 glucan, SDS & Calcium. The activation was dependent upon Ca ions and occurs with limited proteolysis. ß-1, 3 glucan, SDS and Ca activated the peptidase in HLS. Active PO strongly bound to foreign surfaces and this phenomenon may assist in providing opsonic properties for the proPO cascade.
منابع مشابه
Neurosecretory cells of the central nervous system of the freshwater crab, Barytelphusa cunicularis (Westwood 1836).
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تاریخ انتشار 2010